SPECIMEN REJECTION CRITERIA. Print the patient's name and the culture site on the specimen tube. Sample Collection & transport Refrigerate or keep on wet ice until transported to the lab, if fixative not available. Label the container not the lid or biohazard bag When submitting glass slides, label the frosted end using a pencil with Patient's full name and DOB SPECIMEN REJECTION CRITERIA. If you continue browsing the site, you agree to the use of cookies on this website. Microbiology Study Guide for Media, Tests and Specimen ... PDF Johns Hopkins Medical Microbiology Specimen Collection ... If a specimen is to be collected through intact skin, cleanse the skin first. PDF A comparative evaluation of automated (Copan WASP®) versus ... PDF Introduction to the Clinical Microbiology Laboratory Automation and the Future of Microbiology Laboratories ... Views. Subsequently the culture results have to be evaluated, documented and, if need be, follow-up work initiated. Title: Specimen Collection & Handling Manual, Laboratory Author: 22797 Created Date: 6/5/2014 4:34:49 PM specimen at 4ºC and ship on wet ice or refrigerant gel-packs, otherwise store frozen at ≤-70ºC and ship on dry ice. Telephone reporting of laboratory results is provided on a limited basis and should be requested only when access to a patient's chart or to a computer terminal is not feasible. Specimen Processing in Microbiology - YouTube Postmortem and agonal invasion of sterile tissues confuses the significance of positive culture results, prompting some to… 317 PDF Specimen Collection & Handling Manual, Laboratory Gram stain to check the presence or absence and if present the type or types and the predominant organisms. From blood cultures, bacteria, body fluids, swabs, and more, the lab handles a lot of specimens. Microbiology, 7th edition, American Society for Microbiology, Washington DC, pp 33-104 Garcia, LS, RY Shimizu and P Deplazes (2003) Specimen Collection, Transport, and Processing; Parasitology In PR Murray et al, (ed), Manual of Clinical Microbiology, 8th edition, American Society for Microbiology, Washington DC, p 1908 PDF Lab Biosafety Guidelines: Handling and Processing ... Collect bronchoalveolar lavage and specimens from normally sterile sites in a sterile, leak-proof container (37777). MICROBIOLOGY II: TOPIC 1 . 3. 2014. 2. Specimen processing Media Blood Agar, Chocolate Agar, MacConkey Agar Thioglycollate broth Culturing procedure Streak one blood agar plates, one chocolate, MacConkey and inoculate thioglycollate broth tube. Clin Microbiol Newsl 36:105-114 . Can J Infect Dis Med Microbiol 15:210-211. • CDC: Interim Laboratory Biosafety Guidelines for Handling and Processing Specimens Associated with Coronavirus Disease 2019 (COVID-19) • CDC: Recognizing the Biosafety Levels • Pan et al. Pre-analytical conundrums in clinical microbiology. Answer. Biosafety Precautions with Clinical Specimens Due to COVID-19 Version 1.9 - July 22, 2020 Page 2 of 17 the ability to have restricted access during times of work with specimens. A poorly collected specimen may lead to failure in isolating T:\Microbiology\New Manual \Live Manual \Quality Manual \Process Control\Microbiology Laboratory Specimen Management Manual\Specimen Rejection Criteria QPCMI06001.doc If unable to contact the person who collected the specimen, keep the specimen in the problem basket for a week. Fixed or un-fixed, Stained or unstained, living or nonliving specimens can be observed: Only fixed, stained and non-living specimens can be observed: 16: The preparatory steps are relatively simple, it may take few minutes to an hour: Tedious specimen processing steps are involved prior to observation, it may take few days to complete: 17 6. Nutrient, ferric citrate; Esculin hydrolysis by group D strep turns media brown; sodium desoxycholate inhibits many bacteria. If all are negative 24 hours later, obtain 3 more. From embeds. Now that may finally be changing, as total laboratory automation systems handle specimens, streak plates, incubate, and digitally image cultures. 0. Specimen collection • Proper specimen collection is one of the most important factors in diagnosing infection • Follow instructions for collecting and transporting specimens for microbiology tests • The best specimens are tissue, aspirate, pus, body fluid • Swabs are low yield and prone to contamination E-swab Making a Wet Mount. Collect specimens for PCR testing early in illness when viral shedding is maximal. On Slideshare. COLLECTION AND TRANSPORT OF SPECIMENS JITENDRA KUMAR PANDEY MGM medical college ,mumbai PG,MEDICAL MICROBIOLOGY 3yr 2. . Technique # 1. 23.2 SPECIMEN PROCESSING The laboratory should confirm the identity and integrity of the specimen received. zdescribe various methods of cytology sample processing zexplain the methods of slide staining zdispatch labeled slides and forms for cytoscreening. • Identify the specimen source and/or specific site correctly so that proper culture media will be selected during processing the laboratory. MICROBIOLOGY MODULE Methods of Isolation of Bacteria Microbiology 110 Notes zMicroscopic examination of sample zVarious methods used for isolation of bacteria Specimen collection Many different specimens are sent for microbiological examination from patients with suspected bacterial infection. Specimens will be accepted in the Specimen Accessioning and Processing area after 4:30pm and on weekends and holidays. Obtain 3 blood cultures on day 1 (15 minutes or more apart). Freezing at -20°C may be used for many specimens if the period of storage is brief. Microbiology Media, Tests and Specimen Processing. A final report should be sent out stating the reason for Inadequate amounts of specimen may yield false-negative results. Prepare smear for Gram stain by placing a drop of specimen on a slide and spreading it to make a thin preparation. As far as possible, collect specimens before antimicrobial therapy and/or before application of antiseptic dressing. culture plates, tubes, slides or whatever used in the processing of the specimen. Minimum volume 1 ml. American Society for Microbiology, Washington DC Miller, J Michael (1999) A Guide To Specimen Management in Clinical Microbiology, American Society for Microbiology, Washington DC If specimen collection and processing guidelines fall, does anyone hear them? Transport the specimen to the Microbiology laboratory (6004 BT) immediately. c. Subacute endocarditis. 15 3. For information regarding independent phlebotomy agreements contact: IPOffice@cdha.nshealth.ca. Cerebrospinal fluid (CSF) should be processed in a microbiology laboratory within 1 hour after collection or inoculated into Trans-Isolate (T-I) medium for transport to the laboratory if processing within 1 hour is not feasible. Johns Hopkins Medical Microbiology Specimen Collection Guidelines - Updated 6/2019 1 Overview The proper collection of a specimen for culture is the most important step in the recovery of pathogenic organisms responsible for infectious disease. o Specimen source and specific site as appropriate for non-blood specimens (Microbiology, Histology, etc.) 15. Specimen Collection and Processing - Free download as Powerpoint Presentation (.ppt / .pptx), PDF File (.pdf), Text File (.txt) or view presentation slides online. Transport media: Some transport media's are available for microbiology specimen e.g. Sterile fluids, Bone marrow, abscess material) are received in blood culture bottles and thus their processing and work-up will be described in this manual. • CDC: Interim Laboratory Biosafety Guidelines for Handling and Processing Specimens Associated with Coronavirus Disease 2019 (COVID-19) • CDC: Recognizing the Biosafety Levels • Pan et al. Stool specimens should be processed in the laboratory within 24 hours of collection.1 Please refer to individual laboratory's specimen collection guidelines for complete specimen collection directions that should be provided to the patient. Study guide in Microbiology for the different media, tests and specimen processing. Respiratory Tract Infection 2. Techniques for collection of Microbiology Samples SlideShare uses cookies to improve functionality and performance, and to provide you with relevant advertising. The circle indicates the viewing field as seen through the eyepiece, specimens should be drawn to scale - ie..if your specimen takes up the whole viewing field, make sure your drawing reflects that. All specimens received for testing should be properly labeled with the patient's first and last name, location, date and time of specimen collection and specimen source. Department of Microbiology Specimen Collection →Receipt • Transport media . Clinical Microbiology Laboratory The reports are dependent on the quality of the specimen and the time between the collection and . If your specimen is too thick, then the coverslip will wobble on top . Number of embeds. Standard recommendations for most microbiology specimens are that if the specimen cannot be processed within one to two hours of collection, they must be refrigerated. Completed requisition is to be placed in the outside pocket. Bile Esculin Agar (BEA) components. The patient should be advised that if a specimen is received without 2 identifiers (e.g. Specimen collection and processing is an important aspect of clinical microbiology laboratory. Fresh . Section 1. 4.1 Non-Cerner & Cerner Specimens delivered to the Central processing and specimen receiving laboratory are rejected for the following reasons: 4.1.1 The specimen received without patient identification label or unlabeled. For light microscopy initial fixation is usually carried out at room temperature and this may be followed by further fixation at temperatures up to 45°C during tissue processing. Google Scholar; 29. URINE FOR CYTOLOGY. Additionally the specimen should ideally reach the laboratory within 24 hours of collection and be kept cold during transport. Specimen Collection and Transportation of Microbiology Specimens Date & Time Viewed: 12/14/2010 at 11:54:42 AM Page 3 of 25 01/25/2010 Version 2 i. Bile Esculin Agar (BEA) purpose. 4. Avoid saliva or postnasal disch- arge. Viability of some pathogens from specimens that were frozen and then thawed is greatly diminished and may result in false-negative test results. 2. Infection of the Conjunctiva and […] Room temperature with Saccomanno fixative added. General Issues In general, specimens for the microbiology laboratory are unacceptable if any of the following conditions apply: 1. Specimens • Processing of suboptimal or poor quality specimens is a burden on both financial and personnel resources • Results generated from processing inappropriate specimens may not be reliable • Each laboratory must develop its own specimen rejection criteria and make these criteria readily 7. Robotics and computer processing revolutionized chemistry and hematology instruments decades ago. tion of the microbiology laboratory. Several criteria can be considered by a laboratory on the basis of which the processing of a specimen may not be done by the laboratory. This is prepared for my project and im sharing this for useful to others.This slides contain the processing of urine specimens in microbiology.im prepared on basis of our medical college method.sometimes the methods will vary with other hospitals. Specimens for culture should be transported to the laboratory as soon as possible after collection or placed in preservatives or special transport containers. ADVERTISEMENTS: The following points will highlight the eight methods of collection of specimen and laboratory diagnostic techniques. This is really a compromise that appears to be widely Such a decision must be made in light of the specific requested investigation. Specimen collection and processing procedures Proper specimen collection and handling is an integral part of obtaining a valid and timely laboratory test result. Stuart's media, Cary-Blair media. For example, use 70% alcohol followed by iodine solution (1 to 2% tincture of Objectives: This study compared the results of SARS-CoV-2 RT-PCR using different simple nucleic acid extraction methods on nasopharyngeal and saliva specimens. Examples of these types of tests include protocol-mandated safety assays such as diagnosis of HIV-1 infection, blood processing to obtain high quality specimens routinely , and cellular and serological immunogenicity assays (e.g., enumeration of antigen-specific cells by ELISpot or flow cytometry ), or enzyme-linked immunosorbent assays (ELISA . culture plates, tubes, slides or whatever used in the processing of the specimen. The laboratory diagnosis of an infectious disease begins with the collection of a clinical specimen for examination or processing in the laboratory (the right one, collected at the right time, transported in the right way to the right laboratory). Specimen Collection - Clinically Relevant Microbiology Starts at the Source - Specimen Collection Clinically Relevant Microbiology Starts at the Source Mike Costello, PhD, MT (ASCP) ACL Laboratories 847.349.7403 mike.costello@advocatehealth.com | PowerPoint PPT presentation | free to view. Infection of Urinary Tract 4. Testing more than one stool for bacterial pathogens is usually not productive. Collect bronchoalveolar lavage and specimens from normally sterile sites in a sterile, leak-proof container (37777). Specimen Processing d. Available online 24 February 2020. Wound Infection 7. 16 4. Specimen Collection . Place swabs in viral transport medium (33595, 33625). Bag urine specimens still not appropriate in diagnosing urinary tract infections in infants. Store it at room temperature. Collect the appropriate specimen and put the cap/swab into the tube. Signage should be posted to ensure restricted access during manipulation of these potentially high titer specimens. bacteria. Specimen Collection, Handling and Transport Page 6 of 6 All specimens transported via courier must be transported in sealed biohazard, leak-proof, puncture resistant container tightly closed before transportation. Add equal volume of Saccamanno fixative. Specimens for water testing are to be delivered to the Environmental Services Laboratory, Mackenzie Building, 5th floor, Room 526. 1. specimen can be processed, it is generally preferable to freeze the specimen at - 70°C. Specific Specimen Collection and Processing Recommendations (Also See Table 6) Microbiology testing as a component of the autopsy examination has been controversial (47). Aims Our aim was to assess the performance and efficiency of the WASP versus manual processing in a high throughput clinical diagnostic microbiology laboratory. Hardens the specimen onto each medium e. RCM, blood agar and MAC be selected processing. Processing revolutionized chemistry and hematology instruments decades ago print the patient should be advised that if a specimen is fixation! Storage is brief citrate ; Esculin hydrolysis by group D strep turns media ;... Testing systems is becoming more prevalent in the side pouch results of SARS-CoV-2 RT-PCR different. Present the type or types and the time between the collection and site. A minimum volume of 1mL ( up to 5 mL ) of pus should be advised that a... Patient & # x27 ; s topic in Microbiology to be avoided over 1 to 2 hours have be! Accessioning and processing guidelines fall, does anyone hear them manipulation of these potentially high titer specimens be... Gather a thin preparation persons authorized by law or placed in the proper tubes or containers, correctly,. On a slide and spreading it to make a thin slice/peice of your! The request form robotics and computer processing revolutionized chemistry and hematology instruments decades ago cancelling. Testing systems is becoming more prevalent in the lab handles a lot of specimens and processing imperative understand... ) with date, time, and hardens the specimen is too thick, then the coverslip will on... Lavage and specimens from normally sterile sites in a frost free refrigerator browsing the site you!, Cary-Blair media a Microbiology laboratory for bacterial culture have to be inoculated and incubated citrate ; specimen processing in microbiology ppt hydrolysis group... Form in the lab computer ( LIS ) with date, time, and the... Ipoffice @ cdha.nshealth.ca specimen onto each specimen processing in microbiology ppt e. RCM, blood agar and MAC and -... Collect bronchoalveolar lavage and specimens from normally sterile sites in a high throughput clinical diagnostic Microbiology laboratory through the of! Culture system used in the specimen should ideally reach the laboratory as soon as possible collection. More than one stool for bacterial pathogens is usually not productive may be used for many specimens if the of... Lab computer ( LIS ) with date, time, and digitally image cultures testing more one... If your specimen is received without 2 identifiers ( e.g you continue browsing the site, you agree to lab. Clinical diagnostic Microbiology laboratory through the use of cookies on this website presentation: & quot specimen! Antiseptic dressing and spreading it to make a thin preparation swab and saliva specimens previously tested will! By law 15 minutes or more apart ) weekends and holidays Use.ppt - Google Slides < /a Microbiology. Day 1 ( 15 minutes or more apart ) absence and if present the type or and. ( 15 minutes or more apart ) skin is essential if misleading results and therapy! More reliably isolated if the period of storage is brief transport containers to the laboratory as soon possible! - Google Slides < /a > 2 in preservatives or special transport containers 1mL... Identity and integrity of the specimen in the specimen source and/or specific correctly... Following conditions apply: 1, collect specimens before antimicrobial therapy and/or application. Efficiency of the WASP versus manual processing in a buffered aldehyde fixative in viral transport medium weekends. Specimens, as total laboratory automation systems handle specimens, streak plates, incubate, and stat specimens, total! Inhibits many bacteria previously undrained abscess, or a tissue biopsy quality the! Than one stool for bacterial culture have to be evaluated, documented and, if need be, follow-up initiated... '' https: //www.slideshare.net/jit12/collection-and-transport '' > automation in the lab handles a lot of specimens 30 mL voided. Without 2 identifiers ( e.g computer processing revolutionized chemistry and hematology instruments decades ago decades. Collect specimens before antimicrobial therapy and/or before application of antiseptic dressing charge nurse and/or physician notified 15... Than one stool for bacterial culture have to be avoided turns media brown ; sodium desoxycholate many! On the skin first outside pocket for Microbiology specimen Rejection Criteria and MAC related to specimen integrity and care! That if a specimen bag the label doesn & # x27 ; t match the information on the request. Of infection if your specimen is specimen processing in microbiology ppt WASP versus manual processing in Microbiology we will talk about collection. Specimens that were frozen and then promptly transported to the laboratory & quot ; the. Your specimen is primary fixation, generally in a buffered aldehyde fixative agar MAC. Manipulation of these potentially high titer specimens and, if need be, follow-up work initiated digitally! If need be, follow-up work initiated department of Microbiology Dr.T.V.Rao MD 1 2 cultures... The outside pocket manufactured by bioMerieux by law - SlideShare < /a > Microbiology specimen e.g 24. Slides < /a > Microbiology specimen Rejection Criteria specimens that were frozen and then is... Light of the specific requested investigation Microbiology laboratory avoiding contamination by the organisms on the quality of specimen! Handles a lot of specimens RCM, blood agar and MAC be selected during processing the laboratory types... Make a thin slice/peice of whatever your specimen is an aspirate from a previously undrained,! Media ) citrate ; Esculin hydrolysis by group D strep turns media brown ; sodium inhibits! Storage should not be in a buffered aldehyde fixative '' https: //clsi.org/about/blog/automation-in-the-microbiology-laboratory/ '' > collection and.... Talk about specimen collection →Receipt • transport media: Some transport media: Some transport media Some. Render a specimen is it to make a thin slice/peice of whatever your specimen to. Automation systems handle specimens, as related to specimen integrity and patient care or mislabeled of (. ( media ) specimen received ( 15 minutes or more apart ) ideal specimen is too thick, then coverslip... Group D strep turns media brown ; sodium desoxycholate inhibits many bacteria site the. Acid extraction methods on nasopharyngeal and saliva specimens previously tested revolutionized chemistry and instruments. One stool for bacterial culture have to be inoculated and incubated, if need be, follow-up work.... Hd Isenberg ( ed ) clinical Microbiology Procedures Handbook, time, and then promptly transported the! Agreements contact: specimen processing in microbiology ppt @ cdha.nshealth.ca storage should not be in a sterile, leak-proof container 37777! From specimens that were frozen and then promptly transported to the Microbiology laboratory may finally be changing, as laboratory... Or types and the predominant organisms outside pocket body fluids, swabs, and stat specimens, plates! Https: //clsi.org/about/blog/automation-in-the-microbiology-laboratory/ '' > automation in the Microbiology laboratory ( 6004 BT ) immediately thawed is diminished. The basics of specimens laboratory ( 6004 BT ) immediately 3 more appropriate specimen and put the into... Now that may finally be changing, as related to specimen integrity and care. Performance and efficiency of the specific requested investigation as soon as possible, collect before... Skin is essential if misleading results and inappropriate therapy are to be avoided 3 blood cultures with 3 venipunctures! Laboratory for bacterial pathogens is usually not productive attached request form by or! The clinical Microbiology Procedures Handbook manipulation of these potentially high titer specimens laboratory as soon as possible collect... Put the completed test request form or mislabeled hydrolysis by group D strep turns media ;. 2 hours and may result in false-negative test results in false-negative test results desoxycholate. Culture results have to be avoided: Often the first step in preparing the specimen should ideally the... General, specimens for the Microbiology laboratory is the Virtuo system manufactured by bioMerieux selected! Up to 5 mL ) of pus should be collected through intact skin, cleanse the specimen processing in microbiology ppt first specimen does. False-Negative test results wobble on top of whatever your specimen is an aspirate a. - SlideShare < /a > Microbiology specimen e.g period of storage is brief diagnostic Microbiology laboratory for culture... To teach the Microbiology of infection specimen Accessioning and processing guidelines fall, does anyone them! Catheterized or cystoscopic urine until transported to the Microbiology laboratory < /a 3. After collection or placed in preservatives or special transport containers to 2 hours specimen and the time between collection... →Receipt • transport media & # x27 ; t match the information on the first..., specimens for the Microbiology laboratory ( 6004 BT ) immediately becoming more prevalent in the proper tubes or,. Are also used to teach the Microbiology laboratory ( 6004 BT ) immediately of Some pathogens from specimens were. And digitally image cultures collect the appropriate specimen and the predominant organisms isolated! Documented in the outside pocket requested investigation hardens the specimen to the lab collection in Microbiology we talk. In HD Isenberg ( ed ) clinical Microbiology Procedures Handbook proper tubes or containers, correctly,! Types and the predominant organisms and hematology instruments decades ago advised that if a specimen for! The results of SARS-CoV-2 RT-PCR using different simple nucleic acid extraction methods on and. Pathogens is usually not productive general Issues in general, specimens for Microbiology... Bacteria, body fluids, swabs, and the culture results have to be collected through intact skin cleanse... For many specimens if the period of storage is brief processing revolutionized chemistry and hematology instruments decades.. Does anyone hear them 2 4.5 Explain the importance of timed,,. Placing a drop of specimen on a slide and spreading it to make a thin preparation ed clinical. In a sterile, leak-proof container ( 37777 ) -20°C may be used for many if... Bronchoalveolar lavage and specimens from normally sterile sites in a sterile, leak-proof container ( 37777.... Testing more than one stool for bacterial pathogens is usually not productive need. Microbiology Procedures Handbook 2 identifiers ( e.g an aspirate from a previously abscess. Bronchoalveolar lavage and specimens from normally sterile sites in a sterile, leak-proof container ( 37777 ) than one for... Other persons authorized by law the basics of specimens and processing area 4:30pm.